WebSep 15, 2003 · The cHS4 insulator is located at the 5′ boundary between highly compacted chromatin and the transcriptionally active β-globin locus in chicken erythroid cells (1). Like other insulators, cHS4 blocks enhancer function on promoters and protects transgenes from position effects (2,3).
Prototypic chromatin insulator cHS4 protects retroviral transgene …
The cHS4 insulator acts as both a barrier to chromatin-mediated silencing via heterochromatin spreading, and blocks interactions between enhancers and promoters. A distinguishing characteristic of cHS4 is that it has a repetitive heterochromatic region on its 5' end. See more An insulator is a type of cis-regulatory element known as a long-range regulatory element. Found in multicellular eukaryotes and working over distances from the promoter element of the target gene, an insulator is typically … See more Insulators have two main functions: 1. Enhancer-blocking insulators prevent distal enhancers from acting on the promoter of neighbouring genes 2. Barrier insulators prevent silencing of euchromatin by the spread of neighbouring heterochromatin See more The insulator element that is found in the gypsy retrotransposon of Drosophila is one of several sequences that have been studied in detail. The gypsy insulator can be found in the 5' untranslated region (UTR) of the retrotransposon element. Gypsy affects the … See more Imprinting The ability of enhancers to activate imprinted genes is dependent on the presence of an insulator on the unmethylated allele between the two genes. An example of this is the Igf2-H19 imprinted locus. In … See more Enhancer-blocking insulators Similar mechanism of action for enhancer-blocking insulators; chromatin loop domains are … See more The CTCF insulator appears to have enhancer blocking activity via its 3D structure and have no direct connection with barrier activity. … See more The first examples of insulators in vertebrates was seen in the chicken β-globin locus, cHS4. cHS4 marks the border between the active euchromatin in the β-globin locus … See more WebNov 30, 2007 · When cHS4 was located outside the tandem genes, the transcriptional interference was clearly observed . To alleviate transcriptional interference, cHS4 insulator must be located between the two tandem genes. CTCF and USF1 are required for alleviating diminution of the downstream gene expression great white shark maui
Extended core sequences from the cHS4 insulator are …
WebDec 1, 2024 · insulator sequence a2 chs4 aloxe3 a2 mutant chs4 mutant aloxe3 mutant a2 scrambled ... WebJun 7, 2024 · Chicken hypersensitive site-4 ( cHS4) is the first identified insulator in vertebrate genome located at the 5ʹ end of chicken β-globin locus with both enhancer blocking and barrier insulating activities (Chung et al. 1993 ). WebOct 11, 2024 · Insulators are cis-acting regulatory sequences that enhance blocking activity to prevent the spread of heterochromatin and silencing of genes . The chicken hypersensitive site 4 (cHS4) is one of most … great white shark mating season