Cytokinin dehydrogenase activity

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August undefined, 2024

WebThe cytokinin dehydrogenases (CKX; EC 1.5.99.12) are a protein family that maintains the endogenous levels of cytokinins in plants by catalyzing their oxidative degradation. The CKX family in maize (Zea mays L.) has thirteen members, only two of which - ZmCKX1 and ZmCKX10 - have previously been characterized in detail. WebMar 24, 2010 · Almost 80% of 52 regenerated lines of Golden Promise exhibited significantly decreased cytokinin oxidase/dehydrogenase (CKX) enzyme activity in bulked samples of their T 1 roots. There was a positive correlation between the enzyme activity and the plant productivity, expressed as the yield, the number of seeds per plant, and the 1000 grain …

CKX5 cytokinin oxidase 5 [ Arabidopsis thaliana (thale cress) ]

Webcytokinin oxidase activity in the presence of copper-imidazole complexes was first observed by Chatfield and Armstrong [9]. This enhancement was not affected by … WebJun 1, 2024 · The overexpression of CycD3 is sufficient to induce cytokinin-independent shoot formation in the calli 98, and the loss of CycD3 activity reduces the ability of exogenous cytokinins to induce ... csl plasma fort collins pay

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WebThe size and activity of the shoot apical meristem is regulated by transcription factors and low molecular mass signals, including the plant hormone cytokinin. The cytokinin status of the meristem depends on different factors, including metabolic degradation of the hormone, which is catalyzed by cytokinin oxidase/dehydrogenase (CKX) enzymes. WebNov 29, 2024 · Cytokinins have opposing actions in shoot and root growth—actions shown to involve cytokinin dehydrogenase (CKX), … WebCytokinin group of phytohormones regulate root elongation and branching during post‐embryonic development. Cytokinin degrading enzymes cytokinin … eagle rock state park pa

Tissue Localization of Cytokinin Dehydrogenase in Maize: …

Cytokinin oxidase/dehydrogenase inhibitors: outlook for …

WebModifying the cytokinin content in plants is a means of improving plant productivity. Here, we report the development and biological activity of compound TD-K (1-(furan-2 … Webcytokinin, any of a number of plant hormones that influence growth and the stimulation of cell division. Cytokinins are synthesized in the roots and are usually derived from … csl plasma flint michiganWebApr 11, 2024 · 1.Introduction. Zinc (Zn), as one of the essential trace elements, regulates the activity and conformational stability of various proteins and enzymes by acting as a catalyst and regulatory or structural cofactor (Coleman, 1998).In plants, approximately 2,400 proteins require activation by Zn 2+ (Sinclair and Krämer, 2012), such as Zn-finger DNA … eagle rock tax

"WebStrigolactone plays a vital role in plant growth and development, but its response genes remain to be identified. In this study, we found that cytokinin content is markedly increased in the strigolactone signaling mutant d53, and that OsCKX9, which encodes a cytokinin oxidase to catalyze the degradation of cytokinin, functions as a primary strigolactone … " - Cytokinin dehydrogenase activity

Cytokinin dehydrogenase activity

Cytokinin oxidase or dehydrogenase? - FEBS Press

WebMay 6, 2024 · Inhibitors of cytokinin oxidase/dehydrogenase (CKX) reduce the degradation of cytokinins in plants, and this effect can be exploited in agriculture … WebMay 1, 2005 · Fig. 3 Histochemical detection of cytokinin dehydrogenase activity in maize seedlings (2 weeks after germination). (A) Stem cross-section and (B) close-up of phloem cells. Left and right panels show activity staining of the sections with iP substrate and control experiments without iP, respectively. The activity is expressed as purple ...

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WebJun 16, 2024 · a Cytokinin dehydrogenase activity in young panicles of WT and Osckx2-2 mutant. b Cytokinin content in the young panicles of WT and Osckx2-2 mutant. All data represented as Mean ± SD (n = 3). **A significant difference at the 0.01 level by the Student’s t test. Full size image. WebOct 31, 2015 · Cytokinins are hormones that regulate plant development and their environmental responses. Their levels are mainly controlled by the cytokinin oxidase/dehydrogenase (CKO), which oxidatively cleaves cytokinins using redox-active electron acceptors. CKO belongs to the group of flavoproteins with an 8α-N1-histidyl …

WebModifying the cytokinin content in plants is a means of improving plant productivity. Here, we report the development and biological activity of compound TD-K (1-(furan-2-ylmethyl)-3-(1,2,3-thiadiazol-5-yl)urea)which is related to thidiazuron. TD-K—which exhibited extremely high antisenescence activity in the wheat leaf bioassay—and INCYDE (2 … WebApr 26, 2024 · Cytokinin oxidase/dehydrogenase enzymes (CKX) catalyse the degradation of cytokinin by cleaving the active cytokinin form’s unsaturated …

WebMar 29, 2024 · In addition, the degradation of cytokinin mainly depends on the activity of cytokinin oxidative dehydrogenase, which irreversibly inhibits cytokinin degradation (Yeh et al. 2015; Xiao et al. 2016). Morris et al. ( 1999 ) found that cytokinins are extensively degraded in the anthers and pollen of cytokinin oxidative dehydrogenase transgenic …

WebMar 4, 2014 · We have developed a N 6-dimethylallyladenine (cytokinin) dehydrogenase-based microbiosensor for real-time determination of the family of hormones known as cytokinins.Cytokinin dehydrogenase from Zea mays (ZmCKX1) was immobilised concurrently with electrodeposition of a silica gel film on the surface of a Pt …

WebThe present study reviews relevant research pertaining to the biological activity of the CKX enzyme in the context of adapting towards abiotic stresses along with improved grain … csl plasma fort gratiot miWebJun 1, 2024 · Cytokinins play two roles in this process: (1) promoting the growth of meristems to provide a source of stem cells as a prerequisite for leaf initiation and … eagle rock table rock lakeWebEven though, it has already been established since 1980s that the enzyme, cytokinin oxidase/dehydrogenase (CKO/CKX) deactivates cytokinins; the potential applications of manipulating these enzymes have mostly been speculated to have a high potential in the biotechnology industry and spreads to agriculture, horticulture and agroforestry ... eagle rock thrift storeWebSep 16, 2015 · Cytokinin dehydrogenase activity was measured using a modified end-point method described earlier . The reaction mixture (total volume of 0.6 ml in 1.5 ml tube) consisted of 100 mM McIlvaine buffer, pH 5.0 or 7.5, electron acceptor, substrate (dissolved in dimethyl sulfoxide that reached the final concentration of 2.5% in the reaction mixture ... csl plasma frederick mdWebMay 6, 2024 · Inhibitors of cytokinin oxidase/dehydrogenase (CKX) reduce the degradation of cytokinins in plants, and this effect can be exploited in agriculture and in plant tissue culture. In this study, we examine the structure–activity relationship of two series of CKX inhibitors based on diphenylurea. csl plasma fort smithWebcytokinin oxidase activity in the presence of copper-imidazole complexes was first observed by Chatfield and Armstrong [9]. This enhancement was not affected by anaerobic conditions, showing that the copper-imidazole complexes are substitutes for oxygen. Recently, the acti-vation of cytokinin oxidase by some phenolic compounds eagle rock thai foodWebCytokinin dehydrogenase (CKX; EC.1.5.99.12) regulates the level of cytokinin (CK) in plants and is involved in CK regulatory activities. In different plants, a small gene family encodes CKX proteins with varied numbers of members. These genes are expanded in the genome mainly due to segmental duplication events. Despite their biological importance, … csl plasma fort worth texas