Dylight 550 vs pe

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http://docs.abcam.com/pdf/immunology/fluorochrome_guide.pdf

INSTRUCTIONS DyLight Amine-Reactive Dyes - Thermo …

Web10 1040.06 0.139 mg of DyLight 550 NHS . Ester 150,000 MW 2 mg IgG × × = 13.9. μl of DyLight 550 NHS Ester 1 mg 100 μl 0.139 mg of DyLight 550 NHS Ester × = D. Labeling Reaction . 1. To protect reagents from moisture, allow DyLight NHS Esters and DMF to equilibrate to room temperature before opening vials. 2. WebR-PE & APC Labeled Antibodies; Biotin & Streptavidin Conjugates; ... DyLight® 350. Features. Brighter and more photostable than AMCA ... 555, ATTO 550, Cy®3, DyLight® 549, Rhodamine. Features. Brighter than Cy®3 Highly water-soluble Validated in multicolor STORM super-resolution imaging. chippewa lever action

HLA DRB1 Antibody (SPM423) [Alexa Fluor® 488]

WebH9 human embryonic stem (ES) cells were stained with either PE Mouse Anti-SSEA-4 (solid line) or PE mouse IgG3 (A112-3) isotype control (catalog number 559926, dashed line), incubated in the dark for 20 minutes at room temperature and analyzed by flow cytometry. Flow cytometry was performed on a BD FACSCalibur™ System. WebDyLight 550 562 576 4 PE* 496, 546 578 5 APC 650 661 4 Alexa Fluor 647 650 665 4 DyLight 650 654 673 4 PerCP 490 675 2 Alexa Fluor 700 Infrared 702 723 2 * PE is the … WebDyLight 550 is brighter in most applications than TRITC, Cy3 & AlexaFluor 555. All Dylight 550 ... R-PE (Ex Max 496 nm / Em. Peak 578nm) R-phycoerythrin (R-PE) has a … grapefruit league baseball 2021

Secondary Antibodies - Novus Biologicals

Fluorochrome Guide - Leinco Technologies

WebTable of Fluorochromes. This is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy. Within groups, roughly in order of excitation wavelength (families excepted). Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. Web38 rows · PE/Cyanine5: 565: 670: 660/20: 5: PE/Dazzle™ 594: 565: 610: 610/20: 5: PE/Fire™ 700* 565: 695: 670/30: 5: Brilliant Violet 421™ ... Spark Blue™ 550* 516: 540 … grapefruit league ballpark locationsWebDyLight 550 NBP3-11552R: DyLight 594 NBP3-11552DL594: DyLight 650 NBP3-11552C: DyLight 680 NBP3-11552FR: DyLight 755 NBP3-11552IR: FITC NBP3-11552F: HRP NBP3-11552H: Janelia Fluor 549 NBP3-11552JF549: Janelia Fluor 646 NBP3-11552JF646: PE NBP3-11552PE: PerCP NBP3-11552PCP: Unconjugated NBP2-45313-0.1mg: View … chippewa legend of sleeping bear dunes

"WebBoth the absorption and emission properties of the DyLight® fluorochromes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation. Our catalog includes secondary antibodies … " - Dylight 550 vs pe

Dylight 550 vs pe

Fluorochrome Guide - Leinco Technologies

WebAnti-Human Fc DyLight® 550 secondary antibody preadsorbed validated for ICC/IF, Flow Cyt. Cited in peer-reviewed publications. Other DyLight® 550 secondaries available. ... Alkaline Phosphatase Biotin DyLight® 488 DyLight® 594 DyLight® 650 FITC HRP SureLight® PE Unconjugated Unconjugated. You may also be interested in. Primary . … WebDyLight 550 emits vibrant orange-to-red fluorescence for a wide array of fluorescence labeling-based applications. It has a strong absorption at 556nm, high fluorescence at 584nm (extinction coefficient 1.5 x105 cm-1M-1) and high quantum yield. ... with the exception of RPE and PE tandem dyes. For more details on the recommendations …

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WebEach Thermo Scientific DyLight antibody labeling kit contains all the necessary components for three 1-mg labeling reactions of any protein with a molecular weight of 50-150 kDa, … WebAug 8, 2024 · 100%. 1.05K Views. 13 Likes. August 8, 2024. By KFW. 16 Comments. I just compare the Dillon 550 and the Lee Classic Turret press. I see how many rounds that I …

Web10 1040.06 0.139 mg of DyLight 550 NHS . Ester 150,000 MW 2 mg IgG × × = 13.9. μl of DyLight 550 NHS Ester 1 mg 100 μl 0.139 mg of DyLight 550 NHS Ester × = D. … WebDec 22, 2014 · The high fluorescence part of the emission spectra typically spans about 50 nm more than for corresponding Alexa dye, i.e. 125 nm instead of 75 nm. With three colours you get approx. 375 nm of ...

Web62269 DyLight 550-2xPEG NHS Ester, 1mg. 62274 DyLight 650-4xPEG NHS Ester, 1mg. 46601 DyLight 680-4xPEG NHS Ester, 1mg. 62277 DyLight 755-4xPEG NHS Ester, … WebCatalog number: 62262. Thermo Scientific DyLight 550 Amine-Reactive Dye is an NHS ester-activated derivative of high-performance DyLight …

WebDyLight 550 NBP3-11552R: DyLight 594 NBP3-11552DL594: DyLight 650 NBP3-11552C: DyLight 680 NBP3-11552FR: DyLight 755 NBP3-11552IR: FITC NBP3-11552F: HRP NBP3-11552H: Janelia Fluor 549 NBP3-11552JF549: Janelia Fluor 646 NBP3-11552JF646: PE NBP3-11552PE: PerCP NBP3-11552PCP: Unconjugated NBP2-45313-0.1mg: View …

WebDyLight™ 550 has a bright orange to red fluorescence rhodamine derivative dye. This dye can be used to replace Alexa555, TRITC and Cy3. DyLight™ 550 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross ... grapefruit league baseball 2023WebDyLight 550 NBP3-11552R: DyLight 594 NBP3-11552DL594: DyLight 650 NBP3-11552C: DyLight 680 NBP3-11552FR: DyLight 755 NBP3-11552IR: FITC NBP3-11552F: HRP NBP3-11552H: Janelia Fluor 549 NBP3-11552JF549: Janelia Fluor 646 NBP3-11552JF646: PE NBP3-11552PE: PerCP NBP3-11552PCP: Unconjugated NBP2-45313-0.1mg: View … grapefruit knife usesWebBackground. CD63 (cluster of differentiation 63), also known as lysosome associated membrane protein 3 (LAMP-3), is a membrane spanning glycoprotein with a molecular mass ranging from 30-60 kDa that was the first characterized member of the tetraspanin superfamily (1,2). CD63 is ubiquitously expressed and largely present on the cell surface … grapefruit league spring training 2023WebYes, the same experimental set-up can be used. 4. What is the excitation and emission of the DyLight® 550 fluorochrome? The excitation is 562nm and the emission is 576nm. As a comparison, the excitation of DyLight® … grapefruit league facility jobsWebJan 10, 2024 · TRITC is excited with light in the green spectrum with a maximum at 550 nm. Its emission maximum is lying at 573 nm. The bond to proteins (e.g. antibodies) is also based on a reactive isothiocyanate group. ... DyLight 405: 399 nm: 434 nm: DyLight 350: 332 nm: 435 nm: BFP (Blue Fluorescent Protein) 380 nm: 439 nm: Alexa 350: 343 nm: … chippewa library hoursWebStreptavidin conjugated to Dylight®550 quantity ... Streptavidin: SureLight® B-PE;1mg. Binding Moiety:?Streptavidin Recommended Dilutions: IHC 1:100;? WB 1:1000 $ 279.00. #D4-2212. Add to cart Details; SureLight® Streptavidin R-Phycoerythrin (standard-intensity) Important Features of SureLight? SAPE (standard-intensity) Interchangeable with ... grapefruit league baseball mapWebDyLight 550 NBP3-11552R: DyLight 594 NBP3-11552DL594: DyLight 650 NBP3-11552C: DyLight 680 NBP3-11552FR: DyLight 755 NBP3-11552IR: FITC NBP3-11552F: HRP NBP3-11552H: Janelia Fluor 646 NBP3-11552JF646: PE NBP3-11552PE: PerCP NBP3-11552PCP: Unconjugated NBP2-45313-0.1mg: View Available Formulations. Catalog# & … chippewa library chippewa falls wi