Fluxor herg assay h9c2
WebHere, we sought to develop an optical high-throughput assay to monitor surface and total hERG protein expression that would be useful to discern mechanisms underlying LQT2 … WebThe FluxOR™ thallium flux assay performed in two cell lines, U2OS and HEK293, with stable hERG expression can be used to identify compounds that inhibit hERG channel …
Fluxor herg assay h9c2
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WebJul 16, 2024 · H9C2 cells were subcultured under anoxic conditions to mimic myocardial hypoxia and subsequently treated with salidroside. Whole cell patch clamp was … WebThe FluxOR assays take advantage of the well described permeability of potassium channels to thallium (Tl+) ions and the utilization of a new and improved indicator dye …
WebDec 15, 2010 · In the present study, we describe a high-capacity, 384- and 1,536-well plate, functional thallium flux assay for the hERG channel that fulfills these criteria. This assay was optimized and validated using different structural classes of hERG inhibitors. WebMar 1, 2010 · The FluxOR assay was able to identify several known specific inhibitors of Kv7.2/7.3 or hERG, highlighting its potential to identify novel and more efficacious small-molecule modulators.
WebSep 6, 2013 · For ATP depletion assays, H9C2 and RCM’s cells were plated in 96 well plates per the manufacturer’s protocol for 24 hr prior to treatments. For gene expression … WebThe FluxOR assay was performed using the washing method recommended by the supplier, and bottom fluorescence intensity was assessed by end-point readout in the SynergyH1 microplate reader …
WebFluxOR™ Differentially Identifies K+ Channel Antagonists Using FDSS6000 Reaction Time (sec) Z' Score FDSS7000EX Effect of reaction time on z' scores using hERG cells and …
WebThe FluxOR™ II Green Potassium Ion Channel Assay is the next-generation solution for high throughput screening (HTS) of potassium ion channel and transporter activities. The FluxOR II Green assay takes … decatur motor vehicle accident lawyer vimeoWebAfter the incubation period, a baseline recording was collected at 1 Hz for 10 s (excitation 470 ± 20 nm, emission 540 ± 30 nm) followed by a thallium stimulus buffer addition (10 μL/well) and ... decatur morgan west behavior campusWebThe FluxOR™ Potassium Ion Channel Assay is a solution for high throughput screening (HTS) of potassium ion channel and transporter activities. The FluxOR™ assay takes … feathers north scottsdaleWebApr 1, 2010 · To validate the feasibility of the FluxOR™ assay as a screening tool for potassium channels, we went on to test the pharmacology of known potassium channel modulators identified as hits in Figure 2.Based on their well-characterized inhibition of hERG or Kv7.2/7/3, haloperidol, linopiridine, XE991, and cisapride were chosen for further … feathers n woodWebFluxOR Assay Kit (Life Technologies, Carlsbad, CA) following the manufacturers protocol. • Fluorescence signals were elicited by 1mM Tl + and 10 mM K + and detected on FLIPR … feathers oar blades lyricsWeb• The non-homogeneous potassium ion channel assay was run using FluxOR Assay Kit (Life Technologies, Carlsbad, CA) following the manufacturer’s protocol. • All plates were run on FLIPR Tetra Systems using the Calcium Assay setup (Ex 485nm, Em 530nm). Data were acquired at 1 - or 2-second intervals for 140 to 200 seconds. Figure 2. K. V decatur movie theater moviesWebFor high-throughput screening of potassium ion channel and transporter activities we use the FluxOR™ II Green Potassium Ion Channel Assay (Thermo Fisher Cat# F20016) and … decatur movie theater showtimes