Phis2载体
WebbpHis2载体价格1000元,pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为Kanamycin,质粒大小为7207bp。pHis2是酵母单杂交载体的pHis2。 WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog #K1617-1 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ...
Phis2载体
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http://www.bioon.com.cn/doc/showarticle.asp?newsid=96627 Webb17 juni 2014 · 看你用的质粒,估计你用的是clontech system3,宿主菌应该是AH109。. 首先,你要确定一下在转化诱饵质粒pGBKT7-A前,你的空菌AH109是否验证过表型;. 如果验证过表型,那就要考虑AH109的弱HIS3表达了。. 因为bait蛋白内在的DNA结合性质AH109的转化子可能表现出轻微的升高 ...
Webb5 nov. 2015 · pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6, pYCplac22-GFP,酵母载体pAUR123,pRS303TEF,pRS304, pRS305,pRS306,pY13TEF,pY14TEF,pY15TEF,pY16TEF,酵母基因重组表达载体pUG6, pSH47,酵母单杂载体pHISi,pLacZi,pHIS2, pGAD424, 酵母双杂交系统:酿酒酵母Y187,酿酒酵母AH109;质粒pGADT7,pGBKT7;对照质粒pGBKT7 … http://html.rhhz.net/SWJSTB/html/2024-12-110.htm
Webb21 mars 2024 · 苹果腐烂病是苹果生产中最具破坏性的病害。. 在苹果树上添加钾可以有效地控制这种疾病。. 然而,苹果在高钾状态下对苹果腐烂病抗性的潜在机制仍然未知。. 在这里,我们发现高K(HK,9.30 g/kg)苹果组织表现出较高的抗病性。. 当阻断K通道时,阻力受 … http://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf
Webb26 maj 2024 · 载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene, located downstream of a …
Webb3 apr. 2014 · 说明可能是有微弱的自激活性或是 表达的诱饵蛋白诱导了酵母内源性 的组氨酸表达.这种情况可以通过 加入3 来抑制其生长.降低筛选的背景本研究构建了3 个橡胶 树14—3—3 蛋白基因的酵母双杂交 诱饵载体,并对其进行自激活和毒性实验鉴定.自激活实验表 … open ping windows 10Webb20 juni 2024 · 设计载体时参照pHIS2载体的多克隆位点信息(PT3705-5)。2、合成不含5 磷酸盐的双链(参照合成方法)。C、将靶DNA插入pHIS2的多克隆位点1、正反义链核苷酸各0.1g在10l 50mM NaCl中混匀2、核苷酸置于70 5min退火,慢慢冷却至室温(30min)3、20l体系完全酶切0.1g pHIS2。 ipad pro 2020 lowest pricehttp://www.kelei-biology.com/plus/view.php?aid=1071 ipad pro 2020 icloud bypasshttp://www.coolaber.com/usr/2160/File/F/F4671.pdf ipad pro 2020 second handWebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog No. 630304 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ... ipad pro 2020 user guide pdf free downloadWebb8 juni 2014 · 本研究根据植物胚柄特异表达基G564启动子关键序列(GAAAAGCGAA)合成次串联重复序列,并与报告质粒pHIS2.1连接构建诱饵载体pHIS2.1.G564.5A;以纯化 … open pinning wizardWebb9 juli 2024 · pHis2编号载体名称北京华越洋生物VECT2110pHis2pHis2载体基本信息出品公司:Clontech载体名称:pHis2质粒类型:酵母单杂交载体高拷贝/低拷贝:克隆方法:多克隆位 … ipad pro 2020 price south africa